| 59 | 0 | 66 |
| 下载次数 | 被引频次 | 阅读次数 |
目的 探讨miR-29b对人脐静脉内皮细胞（HUVECs）增殖与凋亡的影响。方法 实时定量PCR(q RT-PCR)法检测HUVECs中miR-29b的表达水平；HUVECs分别转染miR-29b mimics和miR-29b inhibitor后，MTT法检测HUVECs增殖活力、Hoechst 33342荧光染色法检测HUVECs的凋亡；生物信息学预测miR-29b的靶基因，双荧光素酶报告基因实验和Mcl-1蛋白表达检测实验验证其靶向关系；Western blot法、MTT法、Hoechst 33342荧光染色法分别检测Mcl-1质粒转染组和Mcl-1质粒与miR-29b mimics共转染组的Mcl-1蛋白表达、HUVECs增殖与凋亡情况。结果 miR-29b表达于HUVECs中；与对照组相比，转染miR-29b mimics的HUVECs增殖活力降低，细胞凋亡数增加，而转染miR-29b inhibitor的HUVECs增殖活力增加，细胞凋亡数减少；Mcl-1被确定是miR-29b的直接靶标；Mcl-1的过表达减弱了miR-29b对HUVECs增殖和凋亡的调控作用。结论 miR-29b通过靶向Mcl-1抑制HUVECs的增殖并促进其凋亡。
Abstract：Objective To investigate the impact of miR-29b on proliferation and apoptosis of human umbilical vein endothelial cells(HUVECs). Methods The expression level of miR-29b in HUVECs were detected by q RT-PCR.miR-29b mimics and inhibitor were transfected into HUVECs,respectively.The proliferation of HUVECs were analyzed using MTT assay, Heochst 33342 staining was used to detect the apoptosis of HUVECs.The target gene of miR-29b was predicted by bioinformatics analysis, the dual-luciferase gene reporter assay and the protein expression of Mcl-1 by Western blotting were applied to verify the targe ring relationship.Western blotting,MTT assay,Heochst 33342 staining were used to detect the protein expression level of Mcl-1,the proliferation and apoptosis in cells transfected with Mcl-1 plasmid or the Co-transfection of Mcl-1 plasmid and miR-29b mimics,respectively. Results miR-29b was expressed in HUVECs.Compared with the Control Group,The proliferation of HUVECs in miR-29b mimics group were decreased,while The amount of Apoptotic HUVECs were increased. The proliferation of HUVECs in miR-29b inhibitor group were increased,whereas The amount of Apoptotic HUVECs were decreased. Mcl-1 was identified as a target of miR-29b that could directly bind to it. Overexpression of Mcl-1 attenuated the regulatory effects of miR-29b on proliferation and apoptosis in HUVECs. Conclusion miR-29b could inhibit the proliferation and promote the apoptosis of HUVECs by targeting Mcl-1.
[1]刘功就，李俊林.颈动脉粥样硬化斑块形成及破裂的血流动力学研究现状[J].内蒙古医学杂志，2023,55(7):860—862,866.
[2]曾高峰.动脉粥样硬化性疾病[J].中南医学科学杂志，2017,(5):433—435.
[3]LIHUA WANG, LEI YANG, TINGTING ZHUANG, et al.Tumor-Derived Exosomal mi R-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2[J].J Immunol Res,2022,2022:4769385.
[4]陈浩，黄智，张帅，等.微小核糖核酸-29b通过靶向髓样细胞白血病-1影响脑缺血/再灌注损伤时神经细胞凋亡[J].介入放射学杂志，2021,30(7):697—702.
[5]杨雁，刘行仁，蒋才玉，等.miR-29b与Mcl-1蛋白表达的关系及其对肺癌细胞增殖和凋亡的影响[J].解放军医药杂志，2018,30(3):39—43.
[6]霍春丽，张兰.微RNA对动脉粥样硬化斑块转录后调控的研究进展[J].中国现代医生，2023,61(23):117—119,135.
[7]QING ZHANG, JIA LIU, HUXINYUE DIAN, et al. Activation of Nrf2/HO-1 signaling:An important molecular mechanism of herbal medicine in the treatment of atherosclerosis via the protection of vascular endothelial cells from oxidative stress[J].J Adv Res,2021,34:43—63.
[8]郑平，陈伟，郑艳.三甲胺-N-氧化物调节肾素-血管紧张素系统诱导人脐静脉内皮细胞炎症的作用机制研究[J].中国现代医生，2022,60(6):28—32,46,197.
[9]BARTEL D P. Micro RNAs:genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116(2):281—297.
[10]AMODIO N ,BELLIZZI D , LEOTTA M,et al.miR-29b induces SOCS-1 expression by promoter demethylation and negatively regulates migration of multiple myeloma and endothelial cells[J]. Cell Cycle,2013,12(23):3650—3662.
[11]ZHU H Q, LI Q, DONG L Y, et al. MicroRNA-29b promotes high-fat diet-stimulated endothelial permeability and apoptosis in apo E knock-out mice by down-regulating MT1expression[J]. Int J Cardiol,2014,176(3):764—770.
[12]伍韵贤，曾昭华，苏子焯，等.MicroRNA-29b在动脉粥样硬化血管中的表达[J].中国医药导报，2013 ,10(10):4—5,8.
[13]BATE-EYA L T, DEN HARTOG I J, VAN DER PLOEG I,et al. High efficacy of the BCL-2 inhibitor ABT199(venetoclax)in BCL-2 high-expressing neuroblastoma cell lines and xenografts and rational for combination with MCL-1 inhibition[J].Oncotarget ,2016,7(19):27946—27958.
[14]FERRER P E, FREDERICK P, GULBIS J M, et al.Translocation of a Bak C-terminus mutant from cytosol to mitochondria to mediate cytochrome Crelease:implications for Bak and Bax apoptotic function[J]. PLoS One, 2012,7(3):e31510.
[15]YEE LIAN TIONG, KHUEN YEN NG, RHUN YIAN KOH,et al.Melatonin inhibits high glucose-induced ox-LDL/LDL expression and apoptosis in human umbilical endothelial cells[J].Horm Mol Biol Clin Investig,2020 ,41(4).
[16]ZHI HUANG, LU LU 3, TIANPENG JIANG, et al.miR-29b affects neurocyte apoptosis by targeting MCL-1 during cerebral ischemia/reperfusion injury[J]. Exp Ther Med,2018,16(4):3399—3404.
[17]JUAN CHEN, YANPING LI, YUN LI, et al.Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2[J].Biomed Res Int,2018,2018:6051407.
基本信息:
DOI：10.16096/J.cnki.nmgyxzz.2025.57.11.001
中图分类号:R543.5
引用信息:
[1]冷超群,唐小妹,段朝霞,等.miR-29b靶向Mcl-1对人脐静脉内皮细胞增殖与凋亡的影响[J].内蒙古医学杂志,2025,57(11):1281-1287.DOI:10.16096/J.cnki.nmgyxzz.2025.57.11.001.
基金信息:
永州市指导性科技计划项目（编号：2023YZ031）